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Image Search Results
Journal: Experimental Biology and Medicine
Article Title: Absence of antibody responses to SARS-CoV-2 N protein in COVID-19 vaccine breakthrough cases
doi: 10.1177/15353702221134097
Figure Lengend Snippet: Study design and sampling time points. Participants included naïve and convalescent individuals given either two or three doses of Pfizer vaccine; a convalescent individual given the J&J vaccine; and unvaccinated convalescent individuals. Viral RNA, antibodies against S or N proteins, and inhibition capacities against the viral receptor-binding domain (RBD) of SARS-CoV-2 were evaluated at T1 (2 weeks post the first dose) and T2 (2 weeks post the second dose) in individual participants. At T3 (24 weeks post the first dose or ~5 months postvaccination) and T4 (36 weeks post the first dose or ~8 months postvaccination), viral RNA, antibodies against S or N proteins of SARS-CoV-2, and neutralizing antibodies were evaluated by live-virus-based microneutralization assay. Since the Delta variant emerged (around T3), we evaluated neutralization capacities against the basal B.1 virus (original SARS-CoV-2) and the Delta variant. The Delta variant became the dominant variant ( ⩾ 99%) as most of our participants were reaching T4, thus, we mainly focused on the Delta variant at this time point. Participants gradually received BNT162b2 booster shots after T3 until the endpoint of our study.
Article Snippet: Viral RNA was detected by (qRT-PCR using FDA-approved Luminex
Techniques: Sampling, Inhibition, Binding Assay, Microneutralization Assay, Variant Assay, Neutralization
Journal: Experimental Biology and Medicine
Article Title: Absence of antibody responses to SARS-CoV-2 N protein in COVID-19 vaccine breakthrough cases
doi: 10.1177/15353702221134097
Figure Lengend Snippet: Reduced neutralizing capacity against SARS-CoV-2 Delta variant at 5 months postvaccination and in unvaccinated convalescent individuals. (a) Correlation between neutralization capacities (presented as EC50: half-maximal effective concentration) against B.1-isolate and Delta variant as evidenced by the Spearman correlation coefficient. (b) Neutralization capacities against B.1-isolate (top panels) and Delta variant (bottom panels) in older Vac_naive individuals as compared to younger Vac_naive individuals. Significance was determined using two-sided Mann–Whitney U tests. (c) Age-adjusted neutralization capacities against Delta (filled circles) compared to those against B.1-isolate (open circles). #: Significance was determined using the two-sided Kruskal–Wallis test followed by a two-stage-step-up Benjamini, Krieger, and Yekutieli false discovery rate method for multiple comparison correction. &: The rank ANCOVA was used to evaluate neutralization capacities across study groups, while controlling for the confounding effect of age. The rank ANCOVA results were presented at F -distribution with p -value. The rank ANCOVA was followed by two-sided Dunnett’s post hoc tests to correct multiple comparisons.
Article Snippet: Viral RNA was detected by (qRT-PCR using FDA-approved Luminex
Techniques: Variant Assay, Neutralization, Concentration Assay, MANN-WHITNEY